Journal: Acta Pharmaceutica Sinica. B

Article Title: Boosting 5-ALA-based photodynamic therapy by a liposomal nanomedicine through intracellular iron ion regulation

doi: 10.1016/j.apsb.2021.03.017

Figure Lengend Snippet: Intracellular iron ion regulating mediated PpIX accumulation and DNA repair enzyme inhibition. (A) The membrance fusion of CLs@DiO and MFLs@DiO (scale bar = 10 μm), the distribution of MFLs@DiI in B16–F10 cells (B) (scale bar = 10 μm). (C) The detection of Fe 2+ after incubating with B16–F10 cells for 4 h (scale bar = 25 μm). (D, E) The transformation of 5-ALA analyzed after incubating with B16–F10 cells for 4 h by CLSM (scale bar = 10 μm) and flow cytometry, respectively. (F) DFO inhibits ALKBH2 repair of m1A in dsDNA by using the DpnII digestion assay and semi-quantitative analysis (G), 1: maker, 2: dsDNA (m1A), 3: dsDNA (m1A)+DpnII, 4: dsDNA (m1A)+ALKBH2+Fe 2+ +DFO + DpnII, 5: dsDNA (m1A)+ALKBH2+Fe 2+ +DpnII. Data are presented as means ± SD ( n = 3). ∗∗∗∗ P < 0.0001, ∗∗ P < 0.01, ∗ P < 0.05.

Article Snippet: The B16–F10 cells were seeded into confocal dish (2 × 10 5 cells per dish) for 24 h of incubation and then treated with control, 5-ALA, DFO, 5-ALA + DFO, CLs@5-ALA/DFO, MFLs@5-ALA/DFO (with the same dose of 5-ALA and DFO: 1 mmol/L and 100 μmol/L respectively) for 4 h. After that, FerroOrange working solution (1 mL, 1 μmol/L) was added to each dish and incubated for a certain period of time, and then the samples were observed directly by a confocal laser-scanning microscopy (CLSM, Leica TCS SP8).

Techniques: Enzyme Inhibition Assay, Transformation Assay, Flow Cytometry

Journal: Acta Pharmaceutica Sinica. B

Article Title: Boosting 5-ALA-based photodynamic therapy by a liposomal nanomedicine through intracellular iron ion regulation

doi: 10.1016/j.apsb.2021.03.017

Figure Lengend Snippet: In vitro enhanced PDT of MFLs@5-ALA/DFO. (A) The production of reactive oxygen species (ROS) after 532 nm laser irradiation by fluorescence microscope (scale bar = 200 μm). (B, C) The analyzed of DNA damage by western blotting and comet assay (scale bar = 200 μm). (D) Cells cytotoxicity of 5-ALA, 5-ALA + DFO, MFLs@5-ALA/DFO. (E) Living and dead cell staining and apoptosis test by Calcein-AM and Propidium Iodide (Calcein-AM/PI) (scale bar = 200 μm). (F) Annexin V/PI staining assays of B16–F10 cells. All of them were incubated with different preparations for 4 h and irradiated with 532 nm laser. Data are presented as means ± SD ( n = 3). ∗∗∗ P < 0.001, ∗∗ P < 0.01, ∗ P < 0.05.

Article Snippet: The B16–F10 cells were seeded into confocal dish (2 × 10 5 cells per dish) for 24 h of incubation and then treated with control, 5-ALA, DFO, 5-ALA + DFO, CLs@5-ALA/DFO, MFLs@5-ALA/DFO (with the same dose of 5-ALA and DFO: 1 mmol/L and 100 μmol/L respectively) for 4 h. After that, FerroOrange working solution (1 mL, 1 μmol/L) was added to each dish and incubated for a certain period of time, and then the samples were observed directly by a confocal laser-scanning microscopy (CLSM, Leica TCS SP8).

Techniques: In Vitro, Irradiation, Fluorescence, Microscopy, Western Blot, Single Cell Gel Electrophoresis, Staining, Incubation

Journal: Applications of Nanomaterials in Human Health

Article Title: Nanomaterials and Their Negative Effects on Human Health

doi: 10.1007/978-981-15-4802-4_13

Figure Lengend Snippet: Impact of nanomaterials on organs/cells/tissues

Article Snippet: 1. , Diaminobutane core (DAB) based generation 3, 4, and 5 dendrimer (diaminobutyric polypropylenimine) for gene delivery , Diaminobutyric polypropylenimine (3-, 4-, and 5-), methoxy PEG (~2 kDa) succinimidyl carboxymethyl esters , Bioware PC-3 M-luc-C6 human prostate adenocarcinoma, bioware B16F10-Luc cells, A431 human epidermoid carcinoma, T98G human glioblastoma, DU145 human prostate carcinoma , – , PEGylated G3 and G4-DAB reduced cytotoxicity of dendrimer. G4-DAB with PEG (2 and 5 kDa) reduced toxicity of dendrimer at lower dose of 20 μg/ml , Somani et al. ( ) .

Techniques: In Vitro, In Vivo, Synthesized, Animal Model, Concentration Assay, Activity Assay, Expressing, Blocking Assay